1IRS

IRS-1 PTB DOMAIN COMPLEXED WITH A IL-4 RECEPTOR PHOSPHOPEPTIDE, NMR, MINIMIZED AVERAGE STRUCTURE

1IRS の概要

• エントリーDOI: 10.2210/pdb1irs/pdb
• 分子名称: IRS-1, IL-4 RECEPTOR PHOSPHOPEPTIDE (2 entities in total)
• 機能のキーワード: phosphotyrosine binding domain (ptb), complex, signal transduction, complex (signal transduction-peptide), complex (signal transduction-peptide) complex, complex (signal transduction/peptide)
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted: P24394
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 13890.05

構造登録者

Zhou, M.-M.,Huang, B.,Olejniczak, E.T.,Meadows, R.P.,Shuker, S.B.,Miyazaki, M.,Trub, T.,Shoelson, S.E.,Feisk, S.W. (登録日: 1996-03-22, 公開日: 1997-05-15, 最終更新日: 2024-10-09)

主引用文献

Zhou, M.M.,Huang, B.,Olejniczak, E.T.,Meadows, R.P.,Shuker, S.B.,Miyazaki, M.,Trub, T.,Shoelson, S.E.,Fesik, S.W.
Structural basis for IL-4 receptor phosphopeptide recognition by the IRS-1 PTB domain.
Nat.Struct.Biol., 3:388-393, 1996

PubMed Abstract: We present the NMR structure of the PTB domain of insulin receptor substrate-1 (IRS-1) complexed to a tyrosine-phosphorylated peptide derived from the IL-4 receptor. Despite the lack of sequence homology and different binding specificity, the overall fold of the protein is similar to that of the Shc PTB domain and closely resembles that of PH domains. However, the PTB domain of IRS-1 is smaller than that of Shc (110 versus 170 residues) and binds to phosphopeptides in a distinct manner. We explain the phosphopeptide binding specificity based on the structure of the complex and results of site-directed mutagenesis experiments.

PubMed: 8599766
DOI: 10.1038/nsb0496-388

実験手法

SOLUTION NMR