1IQA

CRYSTAL STRUCTURE OF THE EXTRACELLULAR DOMAIN OF MOUSE RANK LIGAND

1IQA の概要

• エントリーDOI: 10.2210/pdb1iqa/pdb
• 分子名称: RECEPTOR ACTIVATOR OF NUCLEAR FACTOR KAPPA B LIGAND (2 entities in total)
• 機能のキーワード: homotrimer, beta-strand jellyroll, rankl, rank ligand, rank, cytokine, tnf, bone remodeling, osteoclast differentiation factor
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Isoform 1: Cell membrane; Single-pass type II membrane protein. Isoform 2: Cell membrane; Single-pass type II membrane protein. Isoform 3: Cytoplasm. Tumor necrosis factor ligand superfamily member 11, soluble form: Secreted: O35235
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 54002.06

構造登録者

Ito, S.,Wakabayashi, K.,Ubukata, O.,Hayashi, S.,Okada, F.,Hata, T. (登録日: 2001-07-11, 公開日: 2002-03-13, 最終更新日: 2024-10-16)

主引用文献

Ito, S.,Wakabayashi, K.,Ubukata, O.,Hayashi, S.,Okada, F.,Hata, T.
Crystal structure of the extracellular domain of mouse RANK ligand at 2.2-A resolution.
J.Biol.Chem., 277:6631-6636, 2002

PubMed Abstract: Bone remodeling involves the resorption of bone by osteoclasts and the synthesis of bone matrix by osteoblasts. Receptor activator of NF-kappa B ligand (RANKL, also known as ODF and OPGL), a member of the tumor necrosis factor (TNF) family, triggers osteoclastogenesis by forming a complex with its receptor, RANK. We have determined the crystal structure of the extracellular domain of mouse RANKL at 2.2-A resolution. The structure reveals that the RANKL extracellular domain is trimeric, which was also shown by analytical ultracentrifugation, and each subunit has a beta-strand jellyroll topology like the other members of the TNF family. A comparison of RANKL with TNF beta and TNF-related apoptosis-inducing ligand (TRAIL), whose structures were determined to be in the complex form with their respective receptor, reveals conserved and specific features of RANKL in the TNF superfamily and suggests the presence of key residues of RANKL for receptor binding.

PubMed: 11733492
DOI: 10.1074/jbc.M106525200

実験手法

X-RAY DIFFRACTION (2.2 Å)