1IK1

Solution Structure of an RNA Hairpin from HRV-14

1IK1 の概要

• エントリーDOI: 10.2210/pdb1ik1/pdb
• 分子名称: 5'-R(*GP*GP*UP*AP*CP*UP*AP*UP*GP*UP*AP*CP*CP*A)-3' (1 entity in total)
• 機能のキーワード: a form helix, triloop, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4447.69

構造登録者

Huang, H.,Alexandrov, A.,Chen, X.,Barnes III, T.W.,Zhang, H.,Dutta, K.,Pascal, S.M. (登録日: 2001-05-01, 公開日: 2001-07-18, 最終更新日: 2024-05-01)

主引用文献

Huang, H.,Alexandrov, A.,Chen, X.,Barnes III, T.W.,Zhang, H.,Dutta, K.,Pascal, S.M.
Structure of an RNA hairpin from HRV-14.
Biochemistry, 40:8055-8064, 2001

PubMed Abstract: The 5' noncoding region of the picornaviral genome begins with a cloverleaf which is required for viral replication, due at least in part to an interaction with the viral RNA polymerase as part of a fusion with the predominant viral protease. The necessary region of the cloverleaf has previously been narrowed to a highly conserved stem-loop. The solution structure of a 14-nucleotide RNA hairpin, which is part of the conserved stem-loop from human rhinovirus isotype 14, is presented here. The secondary structure of the hairpin is identical to predictions: a five base pair stem is bounded by a triloop with sequence UAU. However, the fold of the triloop is novel, with stacking of the second loop base onto the closing base pair of the stem, and deviations from A form geometry are introduced into the stem regions bordering the triloop, particularly on the 3' side. These deviations and the associated triloop structure could help to explain the distinct sequence conservation and mutational analysis data observed for the stem region of the hairpin, as compared to a second sequentially similar stem in the intact stem-loop.

PubMed: 11434774
DOI: 10.1021/bi010572b

実験手法

SOLUTION NMR