1IJJ

THE X-RAY CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN RABBIT SKELETAL MUSCLE ACTIN AND LATRUNCULIN A AT 2.85 A RESOLUTION

1IJJ の概要

• エントリーDOI: 10.2210/pdb1ijj/pdb
• 関連するPDBエントリー: 1ATN 1ESV
• 分子名称: ACTIN, ALPHA SKELETAL MUSCLE, MAGNESIUM ION, ADENOSINE-5'-TRIPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: actin, latrunculin, cytoskeleton, contractile protein
• 由来する生物種: Oryctolagus cuniculus (rabbit)
• 細胞内の位置: Cytoplasm, cytoskeleton: P68135
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 86099.98

構造登録者

Vorobiev, S.M.,Bubb, M.R.,Almo, S.C. (登録日: 2001-04-26, 公開日: 2002-04-15, 最終更新日: 2023-08-16)

主引用文献

Bubb, M.R.,Govindasamy, L.,Yarmola, E.G.,Vorobiev, S.M.,Almo, S.C.,Somasundaram, T.,Chapman, M.S.,Agbandje-McKenna, M.,McKenna, R.
Polylysine induces an antiparallel actin dimer that nucleates filament assembly: crystal structure at 3.5-A resolution
J.Biol.Chem., 277:20999-21006, 2002

PubMed Abstract: An antiparallel actin dimer has been proposed to be an intermediate species during actin filament nucleation. We now show that latrunculin A, a marine natural product that inhibits actin polymerization, arrests polylysine-induced nucleation at the level of an antiparallel dimer, resulting in its accumulation. These dimers, when composed of pyrene-labeled actin subunits, give rise to a fluorescent excimer, permitting detection during polymerization in vitro. We report the crystallographic structure of the polylysine-actin-latrunculin A complex at 3.5-A resolution. The non-crystallographic contact is consistent with a dimeric structure and confirms the antiparallel orientation of its subunits. The crystallographic contacts reveal that the mobile DNase I binding loop of one subunit of a symmetry-related antiparallel actin dimer is partially stabilized in the interface between the two subunits of a second antiparallel dimer. These results provide a potential explanation for the paradoxical nucleation of actin filaments that have exclusively parallel subunits by a dimer containing antiparallel subunits.

PubMed: 11932258
DOI: 10.1074/jbc.M201371200

実験手法

X-RAY DIFFRACTION (2.85 Å)