1IJ3

GCN4-pVSL Coiled-coil trimer with Serine at the a(16) position

1IJ3 の概要

• エントリーDOI: 10.2210/pdb1ij3/pdb
• 分子名称: general control protein GCN4 (2 entities in total)
• 機能のキーワード: gcn4 coiled coil, transcription
• 細胞内の位置: Nucleus: P03069
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 12014.03

構造登録者

Akey, D.L.,Malashkevich, V.N.,Kim, P.S. (登録日: 2001-04-24, 公開日: 2001-08-08, 最終更新日: 2024-10-16)

主引用文献

Akey, D.L.,Malashkevich, V.N.,Kim, P.S.
Buried polar residues in coiled-coil interfaces.
Biochemistry, 40:6352-6360, 2001

PubMed Abstract: Coiled coils, estimated to constitute 3-5% of the encoded residues in most genomes, are characterized by a heptad repeat, (abcdefg)(n), where the buried a and d positions form the interface between multiple alpha-helices. Although generally hydrophobic, a substantial fraction ( approximately 20%) of these a- and d-position residues are polar or charged. We constructed variants of the well-characterized coiled coil GCN4-p1 with a single polar residue (Asn, Gln, Ser, or Thr) at either an a or a d position. The stability and oligomeric specificity of each variant were measured, and crystal structures of coiled-coil trimers with threonine or serine at either an a or a d position were determined. The structures show how single polar residues in the interface affect not only local packing, but also overall coiled-coil geometry as seen by changes in the Crick supercoil parameters and core cavity volumes.

PubMed: 11371197
DOI: 10.1021/bi002829w

実験手法

X-RAY DIFFRACTION (1.8 Å)