1IIB

CRYSTAL STRUCTURE OF IIBCELLOBIOSE FROM ESCHERICHIA COLI

1IIB の概要

• エントリーDOI: 10.2210/pdb1iib/pdb
• 分子名称: ENZYME IIB OF THE CELLOBIOSE-SPECIFIC PHOSPHOTRANSFERASE SYSTEM (2 entities in total)
• 機能のキーワード: phosphoenolpyruvate dependent phosphotransferase system, iib enzymes, cysteine phosphorylation, phosphotransferase
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm: P69795
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 22846.90

構造登録者

Van Montfort, R.L.M.,Pijning, T.,Kalk, K.H.,Reizer, J.,Saier, M.H.,Thunnissen, M.M.G.M.,Robillard, G.T.,Dijkstra, B.W. (登録日: 1996-12-23, 公開日: 1997-12-24, 最終更新日: 2024-02-07)

主引用文献

van Montfort, R.L.,Pijning, T.,Kalk, K.H.,Reizer, J.,Saier Jr., M.H.,Thunnissen, M.M.,Robillard, G.T.,Dijkstra, B.W.
The structure of an energy-coupling protein from bacteria, IIBcellobiose, reveals similarity to eukaryotic protein tyrosine phosphatases.
Structure, 5:217-225, 1997

PubMed Abstract: . The bacterial phosphoenolpyruvate-dependent phosphotransferase system (PTS) mediates the energy-driven uptake of carbohydrates and their concomitant phosphorylation. In addition, the PTS is intimately involved in the regulation of a variety of metabolic and transcriptional processes in the bacterium. The multiprotein PTS consists of a membrane channel and at least four cytoplasmic proteins or protein domains that sequentially transfer a phosphoryl group from phosphoenolpyruvate to the transported carbohydrate. Determination of the three-dimensional structure of the IIB enzymes within the multiprotein complex would provide insights into the mechanisms by which they promote efficient transport by the membrane channel IIC protein and phosphorylate the transported carbohydrate on the inside of the cell.

PubMed: 9032081
DOI: 10.1016/S0969-2126(97)00180-9

実験手法

X-RAY DIFFRACTION (1.8 Å)