1IHF

INTEGRATION HOST FACTOR/DNA COMPLEX

1IHF の概要

• エントリーDOI: 10.2210/pdb1ihf/pdb
• 分子名称: DNA (35-MER), DNA (5'-D(*GP*GP*CP*CP*AP*AP*AP*AP*AP*AP*GP*CP*AP*TP*T)-3'), DNA (5'-D(*GP*CP*TP*TP*AP*TP*CP*AP*AP*TP*TP*TP*GP*TP*TP*GP*C P*AP*CP*C)-3'), ... (7 entities in total)
• 機能のキーワード: complex (transcription regulation-dna), transcription factor, beta ribbon motif, dna bending protein, transcription-dna complex, transcription/dna
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 45219.24

構造登録者

Rice, P.A.,Yang, S.-W.,Mizuuchi, K.,Nash, H.A. (登録日: 1996-08-21, 公開日: 1997-02-12, 最終更新日: 2024-02-07)

主引用文献

Rice, P.A.,Yang, S.,Mizuuchi, K.,Nash, H.A.
Crystal structure of an IHF-DNA complex: a protein-induced DNA U-turn.
Cell(Cambridge,Mass.), 87:1295-1306, 1996

PubMed Abstract: Integration host factor (IHF) is a small heterodimeric protein that specifically binds to DNA and functions as an architectural factor in many cellular processes in prokaryotes. Here, we report the crystal structure of IHF complexed with 35 bp of DNA. The DNA is wrapped around the protein and bent by >160 degrees, thus reversing the direction of the helix axis within a very short distance. Much of the bending occurs at two large kinks where the base stacking is interrupted by intercalation of a proline residue. IHF contacts the DNA exclusively via the phosphodiester backbone and the minor groove and relies heavily on indirect readout to recognize its binding sequence. One such readout involves a six-base A tract, providing evidence for the importance of a narrow minor groove.

PubMed: 8980235
DOI: 10.1016/S0092-8674(00)81824-3

実験手法

X-RAY DIFFRACTION (2.5 Å)