1IH7
High-Resolution Structure of Apo RB69 DNA Polymerase
Summary for 1IH7
| Entry DOI | 10.2210/pdb1ih7/pdb |
| Related | 1CLQ 1IG9 1WAJ |
| Descriptor | DNA POLYMERASE, POTASSIUM ION, GUANOSINE, ... (4 entities in total) |
| Functional Keywords | dna polymerase, fingers, palm, thumb, transferase |
| Biological source | Enterobacteria phage RB69 |
| Total number of polymer chains | 1 |
| Total formula weight | 105104.59 |
| Authors | Franklin, M.C.,Wang, J.,Steitz, T.A. (deposition date: 2001-04-18, release date: 2001-06-13, Last modification date: 2023-08-16) |
| Primary citation | Franklin, M.C.,Wang, J.,Steitz, T.A. Structure of the Replicating Complex of a Pol alpha Family DNA Polymerase Cell(Cambridge,Mass.), 105:657-667, 2001 Cited by PubMed Abstract: We describe the 2.6 A resolution crystal structure of RB69 DNA polymerase with primer-template DNA and dTTP, capturing the step just before primer extension. This ternary complex structure in the human DNA polymerase alpha family shows a 60 degrees rotation of the fingers domain relative to the apo-protein structure, similar to the fingers movement in pol I family polymerases. Minor groove interactions near the primer 3' terminus suggest a common fidelity mechanism for pol I and pol alpha family polymerases. The duplex product DNA orientation differs by 40 degrees between the polymerizing mode and editing mode structures. The role of the thumb in this DNA motion provides a model for editing in the pol alpha family. PubMed: 11389835DOI: 10.1016/S0092-8674(01)00367-1 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.21 Å) |
Structure validation
Download full validation report
