1IH7

High-Resolution Structure of Apo RB69 DNA Polymerase

1IH7 の概要

• エントリーDOI: 10.2210/pdb1ih7/pdb
• 関連するPDBエントリー: 1CLQ 1IG9 1WAJ
• 分子名称: DNA POLYMERASE, POTASSIUM ION, GUANOSINE, ... (4 entities in total)
• 機能のキーワード: dna polymerase, fingers, palm, thumb, transferase
• 由来する生物種: Enterobacteria phage RB69
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 105104.59

構造登録者

Franklin, M.C.,Wang, J.,Steitz, T.A. (登録日: 2001-04-18, 公開日: 2001-06-13, 最終更新日: 2023-08-16)

主引用文献

Franklin, M.C.,Wang, J.,Steitz, T.A.
Structure of the Replicating Complex of a Pol alpha Family DNA Polymerase
Cell(Cambridge,Mass.), 105:657-667, 2001

PubMed Abstract: We describe the 2.6 A resolution crystal structure of RB69 DNA polymerase with primer-template DNA and dTTP, capturing the step just before primer extension. This ternary complex structure in the human DNA polymerase alpha family shows a 60 degrees rotation of the fingers domain relative to the apo-protein structure, similar to the fingers movement in pol I family polymerases. Minor groove interactions near the primer 3' terminus suggest a common fidelity mechanism for pol I and pol alpha family polymerases. The duplex product DNA orientation differs by 40 degrees between the polymerizing mode and editing mode structures. The role of the thumb in this DNA motion provides a model for editing in the pol alpha family.

PubMed: 11389835
DOI: 10.1016/S0092-8674(01)00367-1

実験手法

X-RAY DIFFRACTION (2.21 Å)