1IDK

PECTIN LYASE A

1IDK の概要

• エントリーDOI: 10.2210/pdb1idk/pdb
• 分子名称: PECTIN LYASE A (2 entities in total)
• 機能のキーワード: lyase, glycoprotein, multigene family
• 由来する生物種: Aspergillus niger
• 細胞内の位置: Secreted (Probable): Q01172
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37982.19

構造登録者

Mayans, O.,Scott, M.,Connerton, I.,Gravesen, T.,Benen, J.,Visser, J.,Pickersgill, R.,Jenkins, J. (登録日: 1996-10-04, 公開日: 1997-10-15, 最終更新日: 2024-10-30)

主引用文献

Mayans, O.,Scott, M.,Connerton, I.,Gravesen, T.,Benen, J.,Visser, J.,Pickersgill, R.,Jenkins, J.
Two crystal structures of pectin lyase A from Aspergillus reveal a pH driven conformational change and striking divergence in the substrate-binding clefts of pectin and pectate lyases.
Structure, 5:677-689, 1997

PubMed Abstract: Microbial pectin and pectate lyases are virulence factors that degrade the pectic components of the plant cell wall. The homogalacturan backbone of pectin varies in its degree of methylation from the highly methylated and relatively hydrophobic form known as pectin, to the fully demethylated and highly charged form known as pectate. Methylated and demethylated regions of pectin are cleaved by pectin lyase and calcium-dependent pectate lyases, respectively. Protein engineering of lyases specific for particular patterns of methylation, will yield modified pectins of high value to the food and pharmaceutical industries.

PubMed: 9195887
DOI: 10.1016/S0969-2126(97)00222-0

実験手法

X-RAY DIFFRACTION (1.93 Å)