1IDE

ISOCITRATE DEHYDROGENASE Y160F MUTANT STEADY-STATE INTERMEDIATE COMPLEX (LAUE DETERMINATION)

1IDE の概要

• エントリーDOI: 10.2210/pdb1ide/pdb
• 分子名称: ISOCITRATE DEHYDROGENASE, MAGNESIUM ION, ISOCITRIC ACID, ... (5 entities in total)
• 機能のキーワード: oxidoreductase (nad(a)-choh(d))
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 46753.40

構造登録者

Bolduc, J.M.,Dyer, D.H.,Scott, W.G.,Singer, P.,Sweet, R.M.,Koshland Junior, D.E.,Stoddard, B.L. (登録日: 1995-01-18, 公開日: 1996-03-08, 最終更新日: 2024-02-07)

主引用文献

Bolduc, J.M.,Dyer, D.H.,Scott, W.G.,Singer, P.,Sweet, R.M.,Koshland Jr., D.E.,Stoddard, B.L.
Mutagenesis and Laue structures of enzyme intermediates: isocitrate dehydrogenase.
Science, 268:1312-1318, 1995

PubMed Abstract: Site-directed mutagenesis and Laue diffraction data to 2.5 A resolution were used to solve the structures of two sequential intermediates formed during the catalytic actions of isocitrate dehydrogenase. Both intermediates are distinct from the enzyme-substrate and enzyme-product complexes. Mutation of key catalytic residues changed the rate determining steps so that protein and substrate intermediates within the overall reaction pathway could be visualized.

PubMed: 7761851

実験手法

X-RAY DIFFRACTION (2.5 Å)