1ID1

CRYSTAL STRUCTURE OF THE RCK DOMAIN FROM E.COLI POTASSIUM CHANNEL

1ID1 の概要

• エントリーDOI: 10.2210/pdb1id1/pdb
• 分子名称: PUTATIVE POTASSIUM CHANNEL PROTEIN (2 entities in total)
• 機能のキーワード: rck domain, e.coli potassium channel, bk channel, rossmann fold, membrane protein
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cell inner membrane; Multi-pass membrane protein: P31069
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 33492.14

構造登録者

Jiang, Y.,Pico, A.,Cadene, M.,Chait, B.T.,MacKinnon, R. (登録日: 2001-04-02, 公開日: 2001-04-11, 最終更新日: 2024-02-07)

主引用文献

Jiang, Y.,Pico, A.,Cadene, M.,Chait, B.T.,MacKinnon, R.
Structure of the RCK domain from the E. coli K+ channel and demonstration of its presence in the human BK channel.
Neuron, 29:593-601, 2001

PubMed Abstract: The intracellular C-terminal domain structure of a six-transmembrane K+ channel from Escherichia coli has been solved by X-ray crystallography at 2.4 A resolution. The structure is representative of a broad class of domains/proteins that regulate the conductance of K+ (here referred to as RCK domains) in prokaryotic K+ transporters and K+ channels. The RCK domain has a Rossmann-fold topology with unique positions, not commonly conserved among Rossmann-fold proteins, composing a well-conserved salt bridge and a hydrophobic dimer interface. Structure-based amino acid sequence alignments and mutational analysis are used to demonstrate that an RCK domain is also present and is an important component of the gating machinery in eukaryotic large-conductance Ca2+ activated K+ channels.

PubMed: 11301020
DOI: 10.1016/S0896-6273(01)00236-7

実験手法

X-RAY DIFFRACTION (2.4 Å)