1IC1

THE CRYSTAL STRUCTURE FOR THE N-TERMINAL TWO DOMAINS OF ICAM-1

1IC1 の概要

• エントリーDOI: 10.2210/pdb1ic1/pdb
• 分子名称: INTERCELLULAR ADHESION MOLECULE-1, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: icam-1, immunoglobulin fold, cell adhesion, glycoprotein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 44218.94

構造登録者

Casasnovas, J.M.,Stehle, T.,Liu, J.-H.,Wang, J.-H.,Springer, T.A. (登録日: 1998-03-09, 公開日: 1998-06-17, 最終更新日: 2024-10-30)

主引用文献

Casasnovas, J.M.,Stehle, T.,Liu, J.H.,Wang, J.H.,Springer, T.A.
A dimeric crystal structure for the N-terminal two domains of intercellular adhesion molecule-1.
Proc.Natl.Acad.Sci.USA, 95:4134-4139, 1998

PubMed Abstract: The 3.0-A structure of a 190-residue fragment of intercellular adhesion molecule-1 (ICAM-1, CD54) reveals two tandem Ig-superfamily (IgSF) domains. Each of two independent molecules dimerizes identically with a symmetry-related molecule over a hydrophobic interface on the BED sheet of domain 1, in agreement with dimerization of ICAM-1 on the cell surface. The residues that bind to the integrin LFA-1 are well oriented for bivalent binding in the dimer, with the critical Glu-34 residues pointing away from each other on the periphery. Residues that bind to rhinovirus are in the flexible BC and FG loops at the tip of domain 1, and these and the upper half of domain 1 are well exposed in the dimer for docking to virus. By contrast, a residue important for binding to Plasmodium falciparum-infected erythrocytes is in the dimer interface. The presence of A' strands in both domains 1 and 2, conserved hydrogen bonds at domain junctions, and elaborate hydrogen bond networks around the key integrin binding residues in domain 1 make these domains suited to resist tensile forces during adhesive interactions. A subdivision of the intermediate (I) set of IgSF domains is proposed in which domain 1 of ICAM-1 and previously described I set domains belong to the I1 set and domain 2 of ICAM-1, ICAM-2, and vascular cell adhesion molecule-1 belong to the I2 set.

PubMed: 9539702
DOI: 10.1073/pnas.95.8.4134

実験手法

X-RAY DIFFRACTION (3 Å)