1IBR

COMPLEX OF RAN WITH IMPORTIN BETA

Summary for 1IBR

• Entry DOI: 10.2210/pdb1ibr/pdb
• Descriptor: GTP-binding nuclear protein RAN, Importin beta-1 subunit, MAGNESIUM ION, ... (5 entities in total)
• Functional Keywords: small gtpase; nuclear transport receptor, cell cycle, translation
• Biological source: Homo sapiens (human); More
• Cellular location: Nucleus: P62826; Cytoplasm: Q14974
• Total number of polymer chains: 4
• Total formula weight: 152952.48

Authors

Vetter, I.R.,Arndt, A.,Kutay, U.,Goerlich, D.,Wittinghofer, A. (deposition date: 1999-05-14, release date: 1999-06-03, Last modification date: 2023-12-27)

Primary citation

Vetter, I.R.,Arndt, A.,Kutay, U.,Gorlich, D.,Wittinghofer, A.
Structural view of the Ran-Importin beta interaction at 2.3 A resolution
Cell(Cambridge,Mass.), 97:635-646, 1999

PubMed Abstract: Transport receptors of the Importin beta family shuttle between the nucleus and cytoplasm and mediate transport of macromolecules through nuclear pore complexes. They interact specifically with the GTP-binding protein Ran, which in turn regulates their interaction with cargo. Here, we report the three-dimensional structure of a complex between Ran bound to the nonhydrolyzable GTP analog GppNHp and a 462-residue fragment from Importin beta. The structure of Importin beta shows 10 tandem repeats resembling HEAT and Armadillo motifs. They form an irregular crescent, the concave site of which forms the interface with Ran-triphosphate. The importin-binding site of Ran does not overlap with that of the Ran-binding domain of RanBP2.

PubMed: 10367892
DOI: 10.1016/S0092-8674(00)80774-6

Experimental method

X-RAY DIFFRACTION (2.3 Å)