1IBR

COMPLEX OF RAN WITH IMPORTIN BETA

1IBR の概要

• エントリーDOI: 10.2210/pdb1ibr/pdb
• 分子名称: GTP-binding nuclear protein RAN, Importin beta-1 subunit, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: small gtpase; nuclear transport receptor, cell cycle, translation
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: P62826; Cytoplasm: Q14974
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 152952.48

構造登録者

Vetter, I.R.,Arndt, A.,Kutay, U.,Goerlich, D.,Wittinghofer, A. (登録日: 1999-05-14, 公開日: 1999-06-03, 最終更新日: 2023-12-27)

主引用文献

Vetter, I.R.,Arndt, A.,Kutay, U.,Gorlich, D.,Wittinghofer, A.
Structural view of the Ran-Importin beta interaction at 2.3 A resolution
Cell(Cambridge,Mass.), 97:635-646, 1999

PubMed Abstract: Transport receptors of the Importin beta family shuttle between the nucleus and cytoplasm and mediate transport of macromolecules through nuclear pore complexes. They interact specifically with the GTP-binding protein Ran, which in turn regulates their interaction with cargo. Here, we report the three-dimensional structure of a complex between Ran bound to the nonhydrolyzable GTP analog GppNHp and a 462-residue fragment from Importin beta. The structure of Importin beta shows 10 tandem repeats resembling HEAT and Armadillo motifs. They form an irregular crescent, the concave site of which forms the interface with Ran-triphosphate. The importin-binding site of Ran does not overlap with that of the Ran-binding domain of RanBP2.

PubMed: 10367892
DOI: 10.1016/S0092-8674(00)80774-6

実験手法

X-RAY DIFFRACTION (2.3 Å)