1IBM

STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH A MESSENGER RNA FRAGMENT AND COGNATE TRANSFER RNA ANTICODON STEM-LOOP BOUND AT THE A SITE

1IBM の概要

• エントリーDOI: 10.2210/pdb1ibm/pdb
• 関連するPDBエントリー: 1FJF 1FJG 1IBK 1IBL
• 分子名称: 16S RIBOSOMAL RNA, 30S RIBOSOMAL PROTEIN S7, 30S RIBOSOMAL PROTEIN S8, ... (26 entities in total)
• 機能のキーワード: 30s ribosomal subunit, ribosome, a site, decoding, transfer trna, anticodon, stem-loop, messenger rna, mrna, codon
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 794383.96

構造登録者

Ogle, J.M.,Brodersen, D.E.,Clemons Jr., W.M.,Tarry, M.J.,Carter, A.P.,Ramakrishnan, V. (登録日: 2001-03-28, 公開日: 2001-05-04, 最終更新日: 2024-10-30)

主引用文献

Ogle, J.M.,Brodersen, D.E.,Clemons Jr., W.M.,Tarry, M.J.,Carter, A.P.,Ramakrishnan, V.
Recognition of cognate transfer RNA by the 30S ribosomal subunit.
Science, 292:897-902, 2001

PubMed Abstract: Crystal structures of the 30S ribosomal subunit in complex with messenger RNA and cognate transfer RNA in the A site, both in the presence and absence of the antibiotic paromomycin, have been solved at between 3.1 and 3.3 angstroms resolution. Cognate transfer RNA (tRNA) binding induces global domain movements of the 30S subunit and changes in the conformation of the universally conserved and essential bases A1492, A1493, and G530 of 16S RNA. These bases interact intimately with the minor groove of the first two base pairs between the codon and anticodon, thus sensing Watson-Crick base-pairing geometry and discriminating against near-cognate tRNA. The third, or "wobble," position of the codon is free to accommodate certain noncanonical base pairs. By partially inducing these structural changes, paromomycin facilitates binding of near-cognate tRNAs.

PubMed: 11340196
DOI: 10.1126/science.1060612

実験手法

X-RAY DIFFRACTION (3.31 Å)