1IBK

STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH THE ANTIBIOTIC PAROMOMYCIN

Summary for 1IBK

• Entry DOI: 10.2210/pdb1ibk/pdb
• Related: 1FJF 1FJG 1IBL 1IBM
• Descriptor: 16S RIBOSOMAL RNA, 30S RIBOSOMAL PROTEIN S9, 30S RIBOSOMAL PROTEIN S10, ... (25 entities in total)
• Functional Keywords: 30s ribosomal subunit, ribosome, antibiotic, paromomycin, aminoglycoside, decoding
• Biological source: Thermus thermophilus; More
• Total number of polymer chains: 22
• Total formula weight: 788833.81

Authors

Ogle, J.M.,Brodersen, D.E.,Clemons Jr., W.M.,Tarry, M.J.,Carter, A.P.,Ramakrishnan, V. (deposition date: 2001-03-28, release date: 2001-05-04, Last modification date: 2022-12-21)

Primary citation

Ogle, J.M.,Brodersen, D.E.,Clemons Jr., W.M.,Tarry, M.J.,Carter, A.P.,Ramakrishnan, V.
Recognition of cognate transfer RNA by the 30S ribosomal subunit.
Science, 292:897-902, 2001

PubMed Abstract: Crystal structures of the 30S ribosomal subunit in complex with messenger RNA and cognate transfer RNA in the A site, both in the presence and absence of the antibiotic paromomycin, have been solved at between 3.1 and 3.3 angstroms resolution. Cognate transfer RNA (tRNA) binding induces global domain movements of the 30S subunit and changes in the conformation of the universally conserved and essential bases A1492, A1493, and G530 of 16S RNA. These bases interact intimately with the minor groove of the first two base pairs between the codon and anticodon, thus sensing Watson-Crick base-pairing geometry and discriminating against near-cognate tRNA. The third, or "wobble," position of the codon is free to accommodate certain noncanonical base pairs. By partially inducing these structural changes, paromomycin facilitates binding of near-cognate tRNAs.

PubMed: 11340196
DOI: 10.1126/science.1060612

Experimental method

X-RAY DIFFRACTION (3.31 Å)