1IB2

CRYSTAL STRUCTURE OF A PUMILIO-HOMOLOGY DOMAIN

1IB2 の概要

• エントリーDOI: 10.2210/pdb1ib2/pdb
• 関連するPDBエントリー: 1IB3
• 分子名称: PUMILIO 1, BETA-MERCAPTOETHANOL (3 entities in total)
• 機能のキーワード: pumilio-homology domain, puf motif, rna binding protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 40442.66

構造登録者

Wang, X.,Zamore, P.D.,Hall, T.M.T. (登録日: 2001-03-26, 公開日: 2001-05-09, 最終更新日: 2025-03-26)

主引用文献

Wang, X.,Zamore, P.D.,Hall, T.M.
Crystal structure of a Pumilio homology domain.
Mol.Cell, 7:855-865, 2001

PubMed Abstract: Puf proteins regulate translation and mRNA stability by binding sequences in their target RNAs through the Pumilio homology domain (PUM-HD), which is characterized by eight tandem copies of a 36 amino acid motif, the PUM repeat. We have solved the structure of the PUM-HD from human Pumilio1 at 1.9 A resolution. The structure reveals that the eight PUM repeats correspond to eight copies of a single, repeated structural motif. The PUM repeats pack together to form a right-handed superhelix that approximates a half doughnut. The distribution of side chains on the inner and outer faces of this half doughnut suggests that the inner face of the PUM-HD binds RNA while the outer face interacts with proteins such as Nanos, Brain Tumor, and cytoplasmic polyadenylation element binding protein.

PubMed: 11336708
DOI: 10.1016/S1097-2765(01)00229-5

実験手法

X-RAY DIFFRACTION (1.9 Å)