1I8I

CRYSTAL STRUCTURE OF DSFV MR1 IN COMPLEX WITH THE PEPTIDE ANTIGEN OF THE MUTANT EPIDERMAL GROWTH FACTOR RECEPTOR, EGFRVIII, AT ROOM TEMPERATURE

1I8I の概要

• エントリーDOI: 10.2210/pdb1i8i/pdb
• 関連するPDBエントリー: 1i8k
• 分子名称: EPIDERMAL GROWTH FACTOR RECEPTOR ANTIBODY MR1SCFV LIGHT CHAIN, EPIDERMAL GROWTH FACTOR RECEPTOR ANTIBODY MR1SCFV HEAVY CHAIN, EPIDERMAL GROWTH FACTOR RECEPTOR, EGFRVIII PEPTIDE ANTIGEN, ... (4 entities in total)
• 機能のキーワード: antibody-peptide complex, immunoglobulin fold, peptide antigen, type ii' beta turn, immune system
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 26878.92

構造登録者

Landry, R.C.,Klimowicz, A.C.,Lavictoire, S.J.,Borisova, S.,Kottachchi, D.T.,Lorimer, I.A.,Evans, S.V. (登録日: 2001-03-14, 公開日: 2002-03-14, 最終更新日: 2024-11-06)

主引用文献

Landry, R.C.,Klimowicz, A.C.,Lavictoire, S.J.,Borisova, S.,Kottachchi, D.T.,Lorimer, I.A.,Evans, S.V.
Antibody recognition of a conformational epitope in a peptide antigen: Fv-peptide complex of an antibody fragment specific for the mutant EGF receptor, EGFRvIII.
J.Mol.Biol., 308:883-893, 2001

PubMed Abstract: Epitope mapping studies and the determination of the structure to 1.8 A resolution have been carried out for the antigen-binding fragment MR1 in complex with peptide antigen. MR1 is specific for the novel fusion junction of the mutant epidermal growth factor receptor EGFRvIII and has been reported to have a high degree of specificity for the mutant EGFRvIII over the wild-type EGF receptor. The structure of the complex shows that the peptide antigen residue side-chains found by epitope mapping studies to be critical for recognition are accommodated in pockets on the surface of the Fv. However, the most distinctive portion of the peptide antigen, the novel fusion glycine residue, makes no contact to the Fv and does not contribute directly to the epitope. The specificity of MR1 lies in the ability of this glycine residue to assume the restricted conformation needed to form a type II' beta-hairpin turn more easily, and demonstrates that a peptide antigen can be used to generate a conformational epitope.

PubMed: 11352579
DOI: 10.1006/jmbi.2001.4628

実験手法

X-RAY DIFFRACTION (2.4 Å)