1I81

CRYSTAL STRUCTURE OF A HEPTAMERIC LSM PROTEIN FROM METHANOBACTERIUM THERMOAUTOTROPHICUM

1I81 の概要

• エントリーDOI: 10.2210/pdb1i81/pdb
• 関連するPDBエントリー: 1B34 1D3B
• 分子名称: PUTATIVE SNRNP SM-LIKE PROTEIN (2 entities in total)
• 機能のキーワード: curved anti-parallel beta sheet, structural genomics
• 由来する生物種: Methanothermobacter thermautotrophicus
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 64291.07

構造登録者

Collins, B.M.,Harrop, S.J.,Kornfeld, G.D.,Dawes, I.W.,Curmi, P.M.G.,Mabbutt, B.C. (登録日: 2001-03-12, 公開日: 2001-03-28, 最終更新日: 2023-08-09)

主引用文献

Collins, B.M.,Harrop, S.J.,Kornfeld, G.D.,Dawes, I.W.,Curmi, P.M.,Mabbutt, B.C.
Crystal structure of a heptameric Sm-like protein complex from archaea: implications for the structure and evolution of snRNPs.
J.Mol.Biol., 309:915-923, 2001

PubMed Abstract: The Sm/Lsm proteins associate with small nuclear RNA to form the core of small nuclear ribonucleoproteins, required for processes as diverse as pre-mRNA splicing, mRNA degradation and telomere formation. The Lsm proteins from archaea are likely to represent the ancestral Sm/Lsm domain. Here, we present the crystal structure of the Lsm alpha protein from the thermophilic archaeon Methanobacterium thermoautotrophicum at 2.0 A resolution. The Lsm alpha protein crystallizes as a heptameric ring comprised of seven identical subunits interacting via beta-strand pairing and hydrophobic interactions. The heptamer can be viewed as a propeller-like structure in which each blade consists of a seven-stranded antiparallel beta-sheet formed from neighbouring subunits. There are seven slots on the inner surface of the heptamer ring, each of which is lined by Asp, Asn and Arg residues that are highly conserved in the Sm/Lsm sequences. These conserved slots are likely to form the RNA-binding site. In archaea, the gene encoding Lsm alpha is located next to the L37e ribosomal protein gene in a putative operon, suggesting a role for the Lsm alpha complex in ribosome function or biogenesis.

PubMed: 11399068
DOI: 10.1006/jmbi.2001.4693

実験手法

X-RAY DIFFRACTION (2 Å)