1I7H

CRYSTAL STURCUTURE OF FDX

1I7H の概要

• エントリーDOI: 10.2210/pdb1i7h/pdb
• 分子名称: FERREDOXIN, FE2/S2 (INORGANIC) CLUSTER (3 entities in total)
• 機能のキーワード: 2fe-2s, electron transport
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 37561.89

構造登録者

Kakuta, Y.,Horio, T.,Takahashi, Y.,Fukuyama, K. (登録日: 2001-03-09, 公開日: 2002-03-09, 最終更新日: 2024-03-13)

主引用文献

Kakuta, Y.,Horio, T.,Takahashi, Y.,Fukuyama, K.
Crystal structure of Escherichia coli Fdx, an adrenodoxin-type ferredoxin involved in the assembly of iron-sulfur clusters.
Biochemistry, 40:11007-11012, 2001

PubMed Abstract: Escherichia coli ferredoxin (Fdx) is an adrenodoxin-type [2Fe-2S] ferredoxin. Recent genetic analyses show that it has an essential role in the maturation of various iron-sulfur (Fe-S) proteins. Fdx probably functions as a component of the complex machinery responsible for the biogenesis of Fe-S clusters. Its crystal structure was determined by the multiple-wavelength anomalous dispersion method using the iron atoms in the [2Fe-2S] cluster of the protein and then refined to R and R(free) values of 0.255 and 0.278, respectively, at 1.7 A resolution. The structure of Fdx is similar to the structures of bovine adrenodoxin (Adx) and Pseudomonas putida putidaredoxin (Pdx) whose respective root-mean-square deviations of the corresponding Calpha atoms are 1.8 and 2.2 A. This analysis also revealed the structure of the C-terminal residues protruding into the solvent, which is missing in Adx and Pdx. The [2Fe-2S] cluster is located at the edge of the molecule and bonds with the Sgamma atoms of Cys42, Cys48, Cys51, and Cys87. Electrostatic potential analysis showed that the surface of Fdx has two negatively charged areas separated by a hydrophobic lane. One is conserved on the surface of Adx which is an area of interaction with adrenodoxin reductase. Cys46 is located on the molecular surface in the vicinity of the [2Fe-2S] cluster, an indication that it may be involved in Fe-S cluster formation.

PubMed: 11551196
DOI: 10.1021/bi010544t

実験手法

X-RAY DIFFRACTION (1.7 Å)