1I4F

CRYSTAL STRUCTURE OF HLA-A*0201/MAGE-A4-PEPTIDE COMPLEX

Summary for 1I4F

• Entry DOI: 10.2210/pdb1i4f/pdb
• Related: 1qew
• Descriptor: HLA CLASS I HISTOCOMPATIBILITY ANTIGEN, A-2 ALPHA CHAIN, BETA-2-MICROGLOBULIN, MELANOMA-ASSOCIATED ANTIGEN 4, ... (5 entities in total)
• Functional Keywords: major histocompatibility complex, human leukocyte antigen, melanoma-associated antigen, immune system
• Biological source: Homo sapiens (human); More
• Cellular location: Membrane; Single-pass type I membrane protein: P01892; Secreted: P61769
• Total number of polymer chains: 3
• Total formula weight: 45372.37

Authors

Hillig, R.C.,Coulie, P.G.,Stroobant, V.,Saenger, W.,Ziegler, A.,Huelsmeyer, M. (deposition date: 2001-02-21, release date: 2001-07-25, Last modification date: 2024-10-16)

Primary citation

Hillig, R.C.,Coulie, P.G.,Stroobant, V.,Saenger, W.,Ziegler, A.,Hulsmeyer, M.
High-resolution structure of HLA-A*0201 in complex with a tumour-specific antigenic peptide encoded by the MAGE-A4 gene.
J.Mol.Biol., 310:1167-1176, 2001

PubMed Abstract: The heterotrimeric complex of the human major histocompatibity complex (MHC) molecule HLA-A*0201, beta2-microglobulin and the decameric peptide GVYDGREHTV derived from the melanoma antigen (MAGE-A4 protein has been determined by X-ray crystallography at 1.4 A resolution. MAGE-A4 belongs to a family of genes that are specifically expressed in a variety of tumours. MAGE-A4-derived peptides are presented by MHC molecules at the cell surface to cytotoxic T-lymphocytes. As the HLA-A*0201:MAGE-A4 complex occurs only on tumour cells, it is considered to be an appropriate target for immunotherapy. The structure presented here reveals potential epitopes specific to the complex and indicates which peptide residues could be recognised by T-cell receptors. In addition, as the structure could be refined anisotropically, it was possible to describe the movements of the bound peptide in more detail.

PubMed: 11502003
DOI: 10.1006/jmbi.2001.4816

Experimental method

X-RAY DIFFRACTION (1.4 Å)