1I3J

CRYSTAL STRUCTURE OF THE DNA-BINDING DOMAIN OF INTRON ENDONUCLEASE I-TEVI WITH ITS SUBSTRATE

1I3J の概要

• エントリーDOI: 10.2210/pdb1i3j/pdb
• 分子名称: 5'-D(*TP*TP*CP*TP*TP*GP*GP*GP*TP*CP*TP*AP*CP*CP*GP*TP*TP*TP*AP*AP*T)-3', 5'-D(*AP*AP*TP*TP*AP*AP*AP*CP*GP*GP*TP*AP*GP*AP*CP*CP*CP*AP*AP*GP*A)-3', INTRON-ASSOCIATED ENDONUCLEASE 1, ... (5 entities in total)
• 機能のキーワード: protein-dna complex, extended structure, zn-finger, minor groove helix, helix-turn-helix, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Enterobacteria phage T4
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 26191.01

構造登録者

Van Roey, P.,Waddling, C.A.,Fox, K.M.,Belfort, M.,Derbyshire, V. (登録日: 2001-02-15, 公開日: 2001-07-13, 最終更新日: 2024-02-07)

主引用文献

Van Roey, P.,Waddling, C.A.,Fox, K.M.,Belfort, M.,Derbyshire, V.
Intertwined structure of the DNA-binding domain of intron endonuclease I-TevI with its substrate.
EMBO J., 20:3631-3637, 2001

PubMed Abstract: I-TevI is a site-specific, sequence-tolerant intron endonuclease. The crystal structure of the DNA-binding domain of I-TevI complexed with the 20 bp primary binding region of its DNA target reveals an unusually extended structure composed of three subdomains: a Zn finger, an elongated segment containing a minor groove-binding alpha-helix, and a helix-turn-helix. The protein wraps around the DNA, mostly following the minor groove, contacting the phosphate backbone along the full length of the duplex. Surprisingly, while the minor groove-binding helix and the helix-turn- helix subdomain make hydrophobic contacts, the few base-specific hydrogen bonds occur in segments that lack secondary structure and flank the intron insertion site. The multiple base-specific interactions over a long segment of the substrate are consistent with the observed high site specificity in spite of sequence tolerance, while the modular composition of the domain is pertinent to the evolution of homing endonucleases.

PubMed: 11447104
DOI: 10.1093/emboj/20.14.3631

実験手法

X-RAY DIFFRACTION (2.2 Å)