1HVB

CRYSTAL STRUCTURE OF STREPTOMYCES R61 DD-PEPTIDASE COMPLEXED WITH A NOVEL CEPHALOSPORIN ANALOG OF CELL WALL PEPTIDOGLYCAN

Summary for 1HVB

• Entry DOI: 10.2210/pdb1hvb/pdb
• Related: 1CEF 1CEG 3PTE
• Descriptor: D-ALANYL-D-ALANINE CARBOXYPEPTIDASE, 5-{3-(S)-(4-(R)-ACETYLAMINO-4-CARBOXY-BUTYRYLAMINO)-3-[1-(R)-(1-(R)-CARBOXY-ETHYLCARBAMOYL)-ETHYLCARBAMOYL]-PROPYL}-2-( CARBOXY-PHENYLACETYLAMINO-METHYL)-3,6-DIHYDRO-2H-[1,3]THIAZINE-4-CARBOXYLIC ACID (3 entities in total)
• Functional Keywords: protein-cephalosporin complex, hydrolase
• Biological source: Streptomyces sp.
• Total number of polymer chains: 1
• Total formula weight: 38173.35

Authors

McDonough, M.A.,Lee, W.,Silvaggi, N.R.,Mobashery, S.,Kelly, J.A. (deposition date: 2001-01-08, release date: 2001-02-07, Last modification date: 2024-10-30)

Primary citation

Lee, W.,McDonough, M.A.,Kotra, L.,Li, Z.H.,Silvaggi, N.R.,Takeda, Y.,Kelly, J.A.,Mobashery, S.
A 1.2-A snapshot of the final step of bacterial cell wall biosynthesis.
Proc.Natl.Acad.Sci.USA, 98:1427-1431, 2001

PubMed Abstract: The cell wall imparts structural strength and shape to bacteria. It is made up of polymeric glycan chains with peptide branches that are cross-linked to form the cell wall. The cross-linking reaction, catalyzed by transpeptidases, is the last step in cell wall biosynthesis. These enzymes are members of the family of penicillin-binding proteins, the targets of beta-lactam antibiotics. We report herein the structure of a penicillin-binding protein complexed with a cephalosporin designed to probe the mechanism of the cross-linking reaction catalyzed by transpeptidases. The 1.2-A resolution x-ray structure of this cephalosporin bound to the active site of the bifunctional serine type D-alanyl-D-alanine carboxypeptidase/transpeptidase (EC ) from Streptomyces sp. strain R61 reveals how the two peptide strands from the polymeric substrates are sequestered in the active site of a transpeptidase. The structure of this complex provides a snapshot of the enzyme and the bound cell wall components poised for the final and critical cross-linking step of cell wall biosynthesis.

PubMed: 11171967
DOI: 10.1073/pnas.98.4.1427

Experimental method

X-RAY DIFFRACTION (1.17 Å)