1HV8

CRYSTAL STRUCTURE OF A DEAD BOX PROTEIN FROM THE HYPERTHERMOPHILE METHANOCOCCUS JANNASCHII

Summary for 1HV8

• Entry DOI: 10.2210/pdb1hv8/pdb
• Descriptor: PUTATIVE ATP-DEPENDENT RNA HELICASE MJ0669, SULFATE ION (2 entities in total)
• Functional Keywords: helicase, rna-binding protein, atpase, rna binding protein
• Biological source: Methanocaldococcus jannaschii
• Total number of polymer chains: 2
• Total formula weight: 85582.38

Authors

Story, R.M.,Li, H.,Abelson, J.N. (deposition date: 2001-01-08, release date: 2001-02-21, Last modification date: 2024-11-06)

Primary citation

Story, R.M.,Li, H.,Abelson, J.N.
Crystal structure of a DEAD box protein from the hyperthermophile Methanococcus jannaschii.
Proc.Natl.Acad.Sci.USA, 98:1465-1470, 2001

PubMed Abstract: We have determined the structure of a DEAD box putative RNA helicase from the hyperthermophile Methanococcus jannaschii. Like other helicases, the protein contains two alpha/beta domains, each with a recA-like topology. Unlike other helicases, the protein exists as a dimer in the crystal. Through an interaction that resembles the dimer interface of insulin, the amino-terminal domain's 7-strand beta-sheet is extended to 14 strands across the two molecules. Motifs conserved in the DEAD box family cluster in the cleft between domains, and many of their functions can be deduced by mutational data and by comparison with other helicase structures. Several lines of evidence suggest that motif III Ser-Ala-Thr may be involved in binding RNA.

PubMed: 11171974
DOI: 10.1073/pnas.98.4.1465

Experimental method

X-RAY DIFFRACTION (3 Å)