1HV8

CRYSTAL STRUCTURE OF A DEAD BOX PROTEIN FROM THE HYPERTHERMOPHILE METHANOCOCCUS JANNASCHII

1HV8 の概要

• エントリーDOI: 10.2210/pdb1hv8/pdb
• 分子名称: PUTATIVE ATP-DEPENDENT RNA HELICASE MJ0669, SULFATE ION (2 entities in total)
• 機能のキーワード: helicase, rna-binding protein, atpase, rna binding protein
• 由来する生物種: Methanocaldococcus jannaschii
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 85582.38

構造登録者

Story, R.M.,Li, H.,Abelson, J.N. (登録日: 2001-01-08, 公開日: 2001-02-21, 最終更新日: 2024-11-06)

主引用文献

Story, R.M.,Li, H.,Abelson, J.N.
Crystal structure of a DEAD box protein from the hyperthermophile Methanococcus jannaschii.
Proc.Natl.Acad.Sci.USA, 98:1465-1470, 2001

PubMed Abstract: We have determined the structure of a DEAD box putative RNA helicase from the hyperthermophile Methanococcus jannaschii. Like other helicases, the protein contains two alpha/beta domains, each with a recA-like topology. Unlike other helicases, the protein exists as a dimer in the crystal. Through an interaction that resembles the dimer interface of insulin, the amino-terminal domain's 7-strand beta-sheet is extended to 14 strands across the two molecules. Motifs conserved in the DEAD box family cluster in the cleft between domains, and many of their functions can be deduced by mutational data and by comparison with other helicase structures. Several lines of evidence suggest that motif III Ser-Ala-Thr may be involved in binding RNA.

PubMed: 11171974
DOI: 10.1073/pnas.98.4.1465

実験手法

X-RAY DIFFRACTION (3 Å)