1HP7

A 2.1 ANGSTROM STRUCTURE OF AN UNCLEAVED ALPHA-1-ANTITRYPSIN SHOWS VARIABILITY OF THE REACTIVE CENTER AND OTHER LOOPS

1HP7 の概要

• エントリーDOI: 10.2210/pdb1hp7/pdb
• 分子名称: ALPHA-1-ANTITRYPSIN, ZINC ION, BETA-MERCAPTOETHANOL, ... (4 entities in total)
• 機能のキーワード: uncleaved alpha-1-antitrypsin serpin, protein binding
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted. Short peptide from AAT: Secreted, extracellular space, extracellular matrix: P01009
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 44736.44

構造登録者

Kim, S.-J.,Woo, J.-R.,Seo, E.J.,Yu, M.-H.,Ryu, S.-E. (登録日: 2000-12-12, 公開日: 2001-03-14, 最終更新日: 2021-11-10)

主引用文献

Kim, S.,Woo, J.,Seo, E.J.,Yu, M.,Ryu, S.
A 2.1 A resolution structure of an uncleaved alpha(1)-antitrypsin shows variability of the reactive center and other loops.
J.Mol.Biol., 306:109-119, 2001

PubMed Abstract: Serpin (serine protease inhibitor) proteins are involved in diverse physiological processes including inflammation, coagulation, matrix remodeling, and cell differentiation. Deficiency of normal serpin functions leads to various hereditary diseases. Besides their clinical importance, serpin proteins draw much attention due to the large conformational changes that occur upon interaction with proteases. We present here the crystal structure of an uncleaved alpha(1)-antitrypsin determined by the multiple isomorphous replacement method and refined to 2.1 A resolution. The structure, which is the first active serpin structure based on experimental phases, reveals novel conformations in the flexible loops, including the proximal hinge region of the reactive center loop and the surface cavity region in the central beta-sheet, sheet A. The determined loop conformation explains the results of recent mutagenesis studies and provides detailed insights into the protease inhibition mechanism. The high-resolution structure of active alpha(1)-antitrypsin also provides evidence for the existence of localized van-der-Waals strain in the central hydrophobic core.

PubMed: 11178897
DOI: 10.1006/jmbi.2000.4357

実験手法

X-RAY DIFFRACTION (2.1 Å)