1HNS

H-NS (DNA-BINDING DOMAIN)

1HNS の概要

• エントリーDOI: 10.2210/pdb1hns/pdb
• 分子名称: H-NS (1 entity in total)
• 機能のキーワード: histone-like protein h1, dna-binding protein, dna binding protein
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5308.93

構造登録者

Shindo, H.,Iwaki, T.,Ieda, R.,Kurumizaka, H.,Ueguchi, C.,Mizuno, T.,Morikawa, S.,Nakamura, H.,Kuboniwa, H. (登録日: 1995-04-06, 公開日: 1995-07-10, 最終更新日: 2024-05-22)

主引用文献

Shindo, H.,Iwaki, T.,Ieda, R.,Kurumizaka, H.,Ueguchi, C.,Mizuno, T.,Morikawa, S.,Nakamura, H.,Kuboniwa, H.
Solution structure of the DNA binding domain of a nucleoid-associated protein, H-NS, from Escherichia coli.
FEBS Lett., 360:125-131, 1995

PubMed Abstract: The three-dimensional structure of the C-terminal domain (47 residues) obtained from the hydrolysis of H-NS protein with bovine trypsin was determined by NMR measurements and distance geometry calculations. It is composed of an antiparallel beta-sheet, an alpha-helix and a 3(10)-helix which form a hydrophobic core, stabilizing the whole structure. This domain has been found to bind to DNA. Possible DNA binding sites are discussed on the basis of the solution structure of the C-terminal domain of H-NS.

PubMed: 7875316
DOI: 10.1016/0014-5793(95)00079-O

実験手法

SOLUTION NMR