1HMH
THREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME
Summary for 1HMH
| Entry DOI | 10.2210/pdb1hmh/pdb |
| Descriptor | HAMMERHEAD RIBOZYME-RNA STRAND, HAMMERHEAD RIBOZYME-DNA STRAND (2 entities in total) |
| Functional Keywords | dna-rna hammerhead ribozyme, loop, ribozyme |
| Total number of polymer chains | 6 |
| Total formula weight | 44997.76 |
| Authors | Pley, H.W.,Flaherty, K.M.,McKay, D.B. (deposition date: 1995-06-06, release date: 1995-12-07, Last modification date: 2024-02-07) |
| Primary citation | Pley, H.W.,Flaherty, K.M.,McKay, D.B. Three-dimensional structure of a hammerhead ribozyme. Nature, 372:68-74, 1994 Cited by PubMed Abstract: The hammerhead ribozyme is a small catalytic RNA motif made up of three base-paired stems and a core of highly conserved, non-complementary nucleotides essential for catalysis. The X-ray crystallographic structure of a hammerhead RNA-DNA ribozyme-inhibitor complex at 2.6 A resolution reveals that the base-paired stems are A-form helices and that the core has two structural domains. The first domain is formed by the sequence 5'-CUGA following stem I and is a sharp turn identical to the uridine turn of transfer RNA, whereas the second is a non-Watson-Crick three-base-pair duplex with a divalent-ion binding site. The phosphodiester backbone of the DNA inhibitor strand is splayed out at the phosphate 5' to the cleavage site. The structure indicates that the ribozyme may destabilize a substrate strand in order to facilitate twisting of the substrate to allow cleavage of the scissile bond. PubMed: 7969422DOI: 10.1038/372068a0 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.6 Å) |
Structure validation
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