1HMH

THREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME

1HMH の概要

• エントリーDOI: 10.2210/pdb1hmh/pdb
• 分子名称: HAMMERHEAD RIBOZYME-RNA STRAND, HAMMERHEAD RIBOZYME-DNA STRAND (2 entities in total)
• 機能のキーワード: dna-rna hammerhead ribozyme, loop, ribozyme
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 44997.76

構造登録者

Pley, H.W.,Flaherty, K.M.,McKay, D.B. (登録日: 1995-06-06, 公開日: 1995-12-07, 最終更新日: 2024-02-07)

主引用文献

Pley, H.W.,Flaherty, K.M.,McKay, D.B.
Three-dimensional structure of a hammerhead ribozyme.
Nature, 372:68-74, 1994

PubMed Abstract: The hammerhead ribozyme is a small catalytic RNA motif made up of three base-paired stems and a core of highly conserved, non-complementary nucleotides essential for catalysis. The X-ray crystallographic structure of a hammerhead RNA-DNA ribozyme-inhibitor complex at 2.6 A resolution reveals that the base-paired stems are A-form helices and that the core has two structural domains. The first domain is formed by the sequence 5'-CUGA following stem I and is a sharp turn identical to the uridine turn of transfer RNA, whereas the second is a non-Watson-Crick three-base-pair duplex with a divalent-ion binding site. The phosphodiester backbone of the DNA inhibitor strand is splayed out at the phosphate 5' to the cleavage site. The structure indicates that the ribozyme may destabilize a substrate strand in order to facilitate twisting of the substrate to allow cleavage of the scissile bond.

PubMed: 7969422
DOI: 10.1038/372068a0

実験手法

X-RAY DIFFRACTION (2.6 Å)