1HJK

ALKALINE PHOSPHATASE MUTANT H331Q

1HJK の概要

• エントリーDOI: 10.2210/pdb1hjk/pdb
• 分子名称: ALKALINE PHOSPHATASE, ZINC ION, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: alkaline phosphatase, hydrolase, phosphoric monoester, transferase(phospho, alcohol acceptor)
• 由来する生物種: Escherichia coli
• 細胞内の位置: Periplasm: P00634
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 94831.09

構造登録者

Murphy, J.E.,Stec, B.,Ma, L.,Kantrowitz, E.R. (登録日: 1997-05-30, 公開日: 1997-10-15, 最終更新日: 2024-11-13)

主引用文献

Murphy, J.E.,Stec, B.,Ma, L.,Kantrowitz, E.R.
Trapping and visualization of a covalent enzyme-phosphate intermediate.
Nat.Struct.Biol., 4:618-622, 1997

PubMed Abstract: Using a mutant version of E. coli alkaline phosphatase, we succeeded in trapping and determining the structure of the phospho-enzyme intermediate. The X-ray structure also revealed the catalytic water molecule, bound to one of the active site zinc ions, positioned ideally for the apical attack necessary for the hydrolysis of the intermediate.

PubMed: 9253408
DOI: 10.1038/nsb0897-618

実験手法

X-RAY DIFFRACTION (2.3 Å)