1HA7

STRUCTURE OF A LIGHT-HARVESTING PHYCOBILIPROTEIN, C-PHYCOCYANIN FROM SPIRULINA PLATENSIS AT 2.2A RESOLUTION

1HA7 の概要

• エントリーDOI: 10.2210/pdb1ha7/pdb
• 分子名称: C-PHYCOCYANIN ALPHA CHAIN, C-PHYCOCYANIN BETA CHAIN, PHYCOCYANOBILIN, ... (4 entities in total)
• 機能のキーワード: photosynthesis, cyanobacteria, light-harvesting protein, pigment protein, phycocyanobilin, chromophore
• 由来する生物種: SPIRULINA PLATENSIS (CYANOBACTERIUM, BLUE-GREEN ALGA); 詳細
• 細胞内の位置: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side: P72509 P72508
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 450089.53

構造登録者

Padyana, A.K.,Rajashankar, K.R.,Ramakumar, S. (登録日: 2001-03-29, 公開日: 2002-03-28, 最終更新日: 2023-12-13)

主引用文献

Padyana, A.K.,Bhat, V.B.,Madyastha, K.M.,Rajashankar, K.R.,Ramakumar, S.
Crystal Structure of a Light-Harvesting Protein C-Phycocyanin from Spirulina Platensis
Biochem.Biophys.Res.Commun., 282:893-, 2001

PubMed Abstract: The crystal structure of C-phycocyanin, a light-harvesting phycobiliprotein from cyanobacteria (blue-green algae) Spirulina platensis has been solved by molecular replacement technique. The crystals belong to space group P2(1) with cell parameters a = 107.20, b = 115.40, c = 183.04 A; beta = 90.2 degrees. The structure has been refined to a crystallographic R factor of 19.2% (R(free) = 23.9%) using the X-ray diffraction data extending up to 2.2 A resolution. The asymmetric unit of the crystal cell consists of two (alphabeta)6-hexamers, each hexamer being the functional unit in the native antenna rod of cyanobacteria. The molecular structure resembles that of other reported C-phycocyanins. However, the unique form of aggregation of two (alphabeta)6-hexamers in the crystal asymmetric unit, suggests additional pathways of energy transfer in lateral direction between the adjacent hexamers involving beta155 phycocyanobilin chromophores.

PubMed: 11352634
DOI: 10.1006/BBRC.2001.4663

実験手法

X-RAY DIFFRACTION (2.2 Å)