1H8H

Bovine mitochondrial F1-ATPase crystallised in the presence of 5mm AMPPNP

1H8H の概要

• エントリーDOI: 10.2210/pdb1h8h/pdb
• 関連するPDBエントリー: 1BMF 1COW 1E1Q 1E1R 1E79 1EFR 1H8E 1NBM 1QO1
• 分子名称: BOVINE MITOCHONDRIAL F1-ATPASE, ADENOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, ... (9 entities in total)
• 機能のキーワード: atp phosphorylase, atp phosphorylase (h+ transporting), atp synthase, f1fo atp synthase, f1-atpase, hydrolase
• 由来する生物種: BOS TAURUS (CATTLE); 詳細
• 細胞内の位置: Mitochondrion inner membrane : P19483; Mitochondrion: P00829 P05631
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 354127.32

構造登録者

Braig, K.,Menz, R.I.,Montgomery, M.G.,Leslie, A.G.W.,Walker, J.E. (登録日: 2001-02-06, 公開日: 2001-04-15, 最終更新日: 2023-12-13)

主引用文献

Menz, R.I.,Leslie, A.G.W.,Walker, J.E.
The Structure and Nucleotide Occupancy of Bovine Mitochondrial F(1)-ATPase are not Influenced by Crystallisation at High Concentrations of Nucleotide
FEBS Lett., 494:11-, 2001

PubMed Abstract: Analysis of tryptophan mutants of F(1)-ATPase from Escherichia coli [Löbau et al. (1997) FEBS Lett. 404, 15-18] suggested that nucleotide concentrations used to grow crystals for the determination of the structure of bovine F(1)-ATPase [Abrahams et al. (1994) Nature 370, 621-628] would be sufficient to occupy only two catalytic sites, and that higher concentrations of nucleotide would result in all three sites being occupied. We have determined the structure of bovine F(1)-ATPase at 2.9 A resolution with crystals grown in the presence of 5 mM AMPPNP and 5 microM ADP. Similar to previous structures of bovine F(1)-ATPase determined with crystals grown in the presence of lower nucleotide concentrations, only two beta-subunits have bound nucleotide and the third subunit remains empty.

PubMed: 11297725
DOI: 10.1016/S0014-5793(01)02302-X

実験手法

X-RAY DIFFRACTION (2.9 Å)