1H8A

CRYSTAL STRUCTURE OF TERNARY PROTEIN-DNA COMPLEX3

1H8A の概要

• エントリーDOI: 10.2210/pdb1h8a/pdb
• 関連するPDBエントリー: 1H88 1H89 1HJB
• 分子名称: CAAT/ENHANCER BINDING PROTEIN BETA, MYB TRANSFORMING PROTEIN, DNA(5'-(*GP*AP*TP*GP*TP*GP*GP*CP*GP*CP*AP* AP*TP*CP*CP*TP*TP*AP*AP*CP*GP*GP*AP*CP*TP*G)-3'), ... (5 entities in total)
• 機能のキーワード: transcription/dna, protein-dna complex, transcription regulation, bzip, v-myb, c-myb, amv, c/ebp, avian myeloblastosis virus, transforming protein, transcription-dna complex
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 50223.77

構造登録者

Tahirov, T.H.,Ogata, K. (登録日: 2001-01-31, 公開日: 2002-01-28, 最終更新日: 2023-12-13)

主引用文献

Tahirov, T.H.,Sato, K.,Ichikawa-Iwata, E.,Sasaki, M.,Inoue-Bungo, T.,Shiina, M.,Kimura, K.,Takata, S.,Fujikawa, A.,Morii, H.,Kumasaka, T.,Yamamoto, M.,Ishii, S.,Ogata, K.
Mechanism of C-Myb-C/Ebpbeta Cooperation from Separated Sites on a Promoter
Cell(Cambridge,Mass.), 108:57-, 2002

PubMed Abstract: c-Myb, but not avian myeloblastosis virus (AMV) v-Myb, cooperates with C/EBP beta to regulate transcription of myeloid-specific genes. To assess the structural basis for that difference, we determined the crystal structures of complexes comprised of the c-Myb or AMV v-Myb DNA-binding domain (DBD), the C/EBP beta DBD, and a promoter DNA fragment. Within the c-Myb complex, a DNA-bound C/EBP beta interacts with R2 of c-Myb bound to a different DNA fragment; point mutations in v-Myb R2 eliminate such interaction within the v-Myb complex. GST pull-down assays, luciferase trans-activation assays, and atomic force microscopy confirmed that the interaction of c-Myb and C/EBP beta observed in crystal mimics their long range interaction on the promoter, which is accompanied by intervening DNA looping.

PubMed: 11792321
DOI: 10.1016/S0092-8674(01)00636-5

実験手法

X-RAY DIFFRACTION (2.23 Å)