1H6H

Structure of the PX domain from p40phox bound to phosphatidylinositol 3-phosphate

1H6H の概要

• エントリーDOI: 10.2210/pdb1h6h/pdb
• 分子名称: NEUTROPHIL CYTOSOL FACTOR 4, 2-(BUTANOYLOXY)-1-{[(HYDROXY{[2,3,4,6-TETRAHYDROXY-5-(PHOSPHONOOXY)CYCLOHEXYL]OXY}PHOSPHORYL)OXY]METHYL}ETHYL BUTANOATE, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: px domain
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Cytoplasm, cytosol : Q15080
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16959.11

構造登録者

Karathanassis, D.,Bravo, J.,Pacold, M.,Perisic, O.,Williams, R.L. (登録日: 2001-06-15, 公開日: 2001-11-01, 最終更新日: 2024-05-08)

主引用文献

Bravo, J.,Karathanassis, D.,Pacold, C.M.,Pacold, M.E.,Ellson, C.D.,Anderson, K.E.,Butler, J.G.,Lavenir, I.,Perisic, O.,Hawkins, P.T.,Stephens, L.,Williams, R.L.
The Crystal Structure of the Px Domain from P40Phox Bound to Phosphatidylinositol 3-Phosphate
Mol.Cell, 8:829-, 2001

PubMed Abstract: More than 50 human proteins with a wide range of functions have a 120 residue phosphoinositide binding module known as the PX domain. The 1.7 A X-ray crystal structure of the PX domain from the p40(phox) subunit of NADPH oxidase bound to PtdIns(3)P shows that the PX domain embraces the 3-phosphate on one side of a water-filled, positively charged pocket and reveals how 3-phosphoinositide specificity is achieved. A chronic granulomatous disease (CGD)-associated mutation in the p47(phox) PX domain that abrogates PtdIns(3)P binding maps to a conserved Arg that does not directly interact with the phosphoinositide but instead appears to stabilize a critical lipid binding loop. The SH3 domain present in the full-length protein does not affect soluble PtdIns(3)P binding to the p40(phox) PX domain.

PubMed: 11684018
DOI: 10.1016/S1097-2765(01)00372-0

実験手法

X-RAY DIFFRACTION (1.7 Å)