1H4G

Oligosaccharide-binding to family 11 xylanases: both covalent intermediate and mutant-product complexes display 2,5B conformations at the active-centre

1H4G の概要

• エントリーDOI: 10.2210/pdb1h4g/pdb
• 関連するPDBエントリー: 1H4H
• 分子名称: XYLANASE, beta-D-xylopyranose-(1-4)-2-deoxy-2-fluoro-alpha-D-xylopyranose, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: glycoside hydrolase, xylanase, oligosaccharide, transition-state, intermediate, mutant, boat conformation
• 由来する生物種: BACILLUS AGARADHAERENS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47167.75

構造登録者

Sabini, E.,Wilson, K.S.,Danielsen, S.,Schulein, M.,Davies, G.J. (登録日: 2001-05-11, 公開日: 2002-05-09, 最終更新日: 2020-07-29)

• 主引用文献: Sabini, E.,Sulzenbacher, G.,Dauter, M.,Dauter, Z.,Jorgensen, P.L.,Schulein, M.,Dupont, C.,Davies, G.J.,Wilson, K.S.; Catalysis and Specificity in Enzymatic Glycoside Hydrolysis: A 2,5B Conformation for the Glycosyl-Enzyme Intermediate Revealed by the Structure of the Bacillus Agaradhaerens Family 11 Xylanase.; Chem.Biol., 6:483-, 1999; PubMed: 10381409; DOI: 10.1016/S1074-5521(99)80066-0

実験手法

X-RAY DIFFRACTION (1.1 Å)