1H3P

STRUCTURAL CHARACTERISATION OF A MONOCLONAL ANTIBODY SPECIFIC FOR THE PRES1 REGION OF THE HEPATITIS B VIRUS

Replaces:  1GM3

Summary for 1H3P

• Entry DOI: 10.2210/pdb1h3p/pdb
• Descriptor: ANTIBODY FAB FRAGMENT (3 entities in total)
• Functional Keywords: immunoglobulin, antibody fab fragment
• Biological source: MUS MUSCULUS (MOUSE); More
• Total number of polymer chains: 2
• Total formula weight: 49810.64

Authors

Pizarro, J.C.,Vulliez-Le-normand, B.,Riottot, M.M.,Budkowska, A.,Bentley, G.A. (deposition date: 2002-09-12, release date: 2002-09-19, Last modification date: 2024-10-09)

Primary citation

Pizarro, J.C.,Vulliez-Le-Normand, B.,Riottot, M.M.,Budkowska, A.,Bentley, G.A.
Structural and Functional Characterisation of a Monoclonal Antibody Specific for the Pres1 Region of Hepatitis B Virus
FEBS Lett., 509:463-, 2001

PubMed Abstract: The monoclonal antibody 5a19, raised against the ay serotype of hepatitis B virus, binds to the segment of the preS1 region comprising residues 37-43, which is implicated in attachment of the virus to hepatocytes. The dissociation constant, derived from kinetic studies using surface plasmon resonance techniques, is in the low nanomolar range. The nucleotide sequence of the variable domains has been determined and the corresponding germ-line genes have been identified. The three-dimensional structure of the Fab fragment has been determined by X-ray crystallography to 2.6 A resolution.

PubMed: 11749974
DOI: 10.1016/S0014-5793(01)03190-8

Experimental method

X-RAY DIFFRACTION (2.6 Å)