1GXP

PhoB effector domain in complex with pho box DNA.

1GXP の概要

• エントリーDOI: 10.2210/pdb1gxp/pdb
• 関連するPDBエントリー: 1B00 1GXQ 1QQI
• 分子名称: PHOSPHATE REGULON TRANSCRIPTIONAL REGULATORY PROTEIN, 5'-D(*GP*AP*GP*CP*TP*GP*TP*CP*AP*TP* AP*AP*AP*GP*TP*TP*GP*TP*CP*AP*CP*GP*G)-3', 5'-D(*CP*CP*CP*GP*TP*GP*AP*CP*AP*AP* CP*TP*TP*TP*AP*TP*GP*AP*CP*AP*GP*CP*T)-3', ... (4 entities in total)
• 機能のキーワード: transcriptional activator, helix-winged-helix, sensory transduction, phosphorylation, dna binding, activator, two- component signal transduction
• 由来する生物種: ESCHERICHIA COLI
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 77674.66

構造登録者

Blanco, A.G.,Sola, M.,Gomis-Ruth, F.X.,Coll, M. (登録日: 2002-04-08, 公開日: 2002-04-29, 最終更新日: 2024-05-08)

主引用文献

Blanco, A.G.,Sola, M.,Gomis-Ruth, F.X.,Coll, M.
Tandem DNA Recognition by Two-Component Signal Transduction Transcriptional Activator Phob
Structure, 10:701-, 2002

PubMed Abstract: PhoB is a signal transduction response regulator that activates nearly 40 genes in phosphate depletion conditions in E. coli and closely related bacteria. The structure of the PhoB effector domain in complex with its target DNA sequence, or pho box, reveals a novel tandem arrangement in which several monomers bind head to tail to successive 11-base pair direct-repeat sequences, coating one face of a smoothly bent double helix. The protein has a winged helix fold in which the DNA recognition elements comprise helix alpha 3, penetrating the major groove, and a beta hairpin wing interacting with a compressed minor groove via Arg219, tightly sandwiched between the DNA sugar backbones. The transactivation loops protrude laterally in an appropriate orientation to interact with the RNA polymerase sigma(70) subunit, which triggers transcription initiation.

PubMed: 12015152
DOI: 10.1016/S0969-2126(02)00761-X

実験手法

X-RAY DIFFRACTION (2.5 Å)