1GX7

Best model of the electron transfer complex between cytochrome c3 and [Fe]-hydrogenase

1GX7 の概要

• エントリーDOI: 10.2210/pdb1gx7/pdb
• 関連するPDBエントリー: 1A2I 1HFE 1MDV 2CTH 2CYM
• 分子名称: PERIPLASMIC [FE] HYDROGENASE LARGE SUBUNIT, PERIPLASMIC [FE] HYDROGENASE SMALL SUBUNIT, CYTOCHROME C3, ... (9 entities in total)
• 機能のキーワード: oxidoreductase, electron transfer complex, hydrogenase, multiheme cytochrome, soft docking, oxidoreductase electron transport, 4fe-4s, iron-sulfur
• 由来する生物種: DESULFOVIBRIO VULGARIS; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 65866.20

構造登録者

Elantak, L.,Morelli, X.,Bornet, O.,Hatchikian, C.,Czjzek, M.,Dolla, A.,Guerlesquin, F. (登録日: 2002-03-28, 公開日: 2003-07-31, 最終更新日: 2024-11-06)

主引用文献

Elantak, L.,Morelli, X.,Bornet, O.,Hatchikian, C.,Czjzek, M.,Dolla, A.,Guerlesquin, F.
The Cytochrome C(3)-[Fe]-Hydrogenase Electron-Transfer Complex: Structural Model by NMR Restrained Docking
FEBS Lett., 548:1-, 2003

PubMed Abstract: Cytochrome c(3) (M(r) 13000) is a low redox potential cytochrome specific of the anaerobic metabolism in sulfate-reducing bacteria. This tetrahemic cytochrome is an intermediate between the [Fe]-hydrogenase and the cytochrome Hmc in Desulfovibrio vulgaris Hildenborough strain. The present work describes the structural model of the cytochrome c(3)-[Fe]-hydrogenase complex obtained by nuclear magnetic resonance restrained docking. This model connects the distal cluster of the [Fe]-hydrogenase to heme 4 of the cytochrome, the same heme found in the interaction with cytochrome Hmc. This result gives evidence that cytochrome c(3) is an electron shuttle between the periplasmic hydrogenase and the Hmc membrane-bound complex.

PubMed: 12885397
DOI: 10.1016/S0014-5793(03)00718-X

実験手法

SOLUTION NMR
THEORETICAL MODEL