1GRZ
A PREORGANIZED ACTIVE SITE IN THE CRYSTAL STRUCTURE OF THE TETRAHYMENA RIBOZYME
Summary for 1GRZ
| Entry DOI | 10.2210/pdb1grz/pdb |
| Descriptor | LSU R-RNA GROUP I INTRON (1 entity in total) |
| Functional Keywords | ribozyme structure, rrna group i intron, ribozyme |
| Biological source | Tetrahymena thermophila |
| Total number of polymer chains | 2 |
| Total formula weight | 159792.77 |
| Authors | Golden, B.L.,Gooding, A.R.,Podell, E.R.,Cech, T.R. (deposition date: 1998-09-14, release date: 1998-11-24, Last modification date: 2024-02-07) |
| Primary citation | Golden, B.L.,Gooding, A.R.,Podell, E.R.,Cech, T.R. A preorganized active site in the crystal structure of the Tetrahymena ribozyme. Science, 282:259-264, 1998 Cited by PubMed Abstract: Group I introns possess a single active site that catalyzes the two sequential reactions of self-splicing. An RNA comprising the two domains of the Tetrahymena thermophila group I intron catalytic core retains activity, and the 5.0 angstrom crystal structure of this 247-nucleotide ribozyme is now described. Close packing of the two domains forms a shallow cleft capable of binding the short helix that contains the 5' splice site. The helix that provides the binding site for the guanosine substrate deviates significantly from A-form geometry, providing a tight binding pocket. The binding pockets for both the 5' splice site helix and guanosine are formed and oriented in the absence of these substrates. Thus, this large ribozyme is largely preorganized for catalysis, much like a globular protein enzyme. PubMed: 9841391DOI: 10.1126/science.282.5387.259 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (5 Å) |
Structure validation
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