1GQU

Crystal structure of an alternating A-T oligonucleotide fragment with Hoogsteen base pairing

1GQU の概要

• エントリーDOI: 10.2210/pdb1gqu/pdb
• 分子名称: DNA (5'(*AP*TP*AP*UP*AP*T)-3') (2 entities in total)
• 機能のキーワード: dna, dna/rna, new dna structure, hoogsteen base pairing
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 7488.44

構造登録者

Abrescia, N.G.A.,Thompson, A.,Huynh-Dinh, T.,Subirana, J.A. (登録日: 2001-12-05, 公開日: 2002-12-05, 最終更新日: 2024-05-08)

主引用文献

Abrescia, N.G.A.,Thompson, A.,Huynh-Dinh, T.,Subirana, J.A.
Crystal Structure of an Antiparallel DNA Fragment with Hoogsteen Base Pairing
Proc.Natl.Acad.Sci.USA, 99:2806-, 2002

PubMed Abstract: We report here an alternative double-helical structure of the DNA molecule. It has been found in the d(ATA(Br)UAT) and d(ATATAT) sequences by single-crystal x-ray crystallography. This sequence is found not only in TATA boxes, but also in other regulatory regions of DNA. Bases of the two antiparallel strands form Hoogsteen pairs, with adenines in the syn conformation. The structure is related neither to those found in triple helices nor to parallel DNA duplexes. Its conformational parameters are very similar to those of duplex DNA in the B form. Both forms may coexist under physiological conditions, although the Hoogsteen pairing greatly influences the recognition sites on DNA. Our results demonstrate that an alternative to the classical B-DNA double helix is possible.

PubMed: 11880632
DOI: 10.1073/PNAS.052675499

実験手法

X-RAY DIFFRACTION (2.5 Å)