1GO3

Structure of an archeal homolog of the eukaryotic RNA polymerase II RPB4/RPB7 complex

Summary for 1GO3

• Entry DOI: 10.2210/pdb1go3/pdb
• Descriptor: DNA-DIRECTED RNA POLYMERASE SUBUNIT E, DNA-DIRECTED RNA POLYMERASE SUBUNIT F (3 entities in total)
• Functional Keywords: transferase, transferase; transcription; dna-directed rna polymerase
• Biological source: METHANOCOCCUS JANNASCHII; More
• Total number of polymer chains: 4
• Total formula weight: 67177.68

Authors

Todone, F.,Brick, P.,Werner, F.,Weinzierl, R.O.J.,Onesti, S. (deposition date: 2001-10-17, release date: 2001-12-07, Last modification date: 2024-05-08)

Primary citation

Todone, F.,Brick, P.,Werner, F.,Weinzierl, R.O.J.,Onesti, S.
Structure of an Archaeal Homolog of the Eukaryotic RNA Polymerase II Rpb4/Rpb7 Complex
Mol.Cell, 8:1137-1143, 2001

PubMed Abstract: The eukaryotic subunits RPB4 and RPB7 form a heterodimer that reversibly associates with the RNA polymerase II core and constitute the only two components of the enzyme for which no structural information is available. We have determined the crystal structure of the complex between the Methanococcus jannaschii subunits E and F, the archaeal homologs of RPB7 and RPB4. Subunit E has an elongated two-domain structure and contains two potential RNA binding motifs, while the smaller F subunit wraps around one side of subunit E, at the interface between the two domains. We propose a model for the interaction between RPB4/RPB7 and the core RNA polymerase in which the RNA binding face of RPB7 is positioned to interact with the nascent RNA transcript.

PubMed: 11741548
DOI: 10.1016/S1097-2765(01)00379-3

Experimental method

X-RAY DIFFRACTION (1.75 Å)