1GL2

Crystal structure of an endosomal SNARE core complex

1GL2 の概要

• エントリーDOI: 10.2210/pdb1gl2/pdb
• 分子名称: ENDOBREVIN, SYNTAXIN 7, VESICLE TRANSPORT V-SNARE PROTEIN VTI1-LIKE 1, ... (5 entities in total)
• 機能のキーワード: membrane protein, membrane fusion protein complex, coiled coil, transmembrane, transport
• 由来する生物種: RATTUS NORVEGICUS (RAT); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 29583.63

構造登録者

Antonin, W.,Becker, S.,Jahn, R.,Schneider, T.R. (登録日: 2001-08-22, 公開日: 2002-01-15, 最終更新日: 2023-12-13)

主引用文献

Antonin, W.,Fasshauer, D.,Becker, S.,Jahn, R.,Schneider, T.R.
Crystal Structure of the Endosomal Snare Complex Reveals Common Structural Principles of All Snares.
Nat.Struct.Biol., 9:107-, 2001

PubMed Abstract: SNARE proteins are crucial for intracellular membrane fusion in all eukaryotes. These proteins assemble into tight complexes that connect membranes and may induce fusion. The crystal structure of the neuronal core complex is represented by an unusually long bundle of four alpha-helices connected by 16 layers of mostly hydrophobic amino acids. Here we report the 1.9 A resolution crystal structure of an endosomal SNARE core complex containing four SNAREs: syntaxin 7, syntaxin 8, vti1b and endobrevin/VAMP-8. Despite limited sequence homology, the helix alignment and the layer structure of the endosomal complex are remarkably similar to those of the neuronal complex. However, subtle variations are evident that characterize different SNARE subfamilies. We conclude that the structure of the SNARE core complex is an evolutionarily conserved hallmark of all SNARE complexes and is intimately associated with the general role of SNAREs in membrane fusion.

PubMed: 11786915
DOI: 10.1038/NSB746

実験手法

X-RAY DIFFRACTION (1.9 Å)