1GKU

Reverse gyrase from Archaeoglobus fulgidus

1GKU の概要

• エントリーDOI: 10.2210/pdb1gku/pdb
• 分子名称: REVERSE GYRASE (2 entities in total)
• 機能のキーワード: topoisomerase, dna supercoiling, archaea, helicase
• 由来する生物種: ARCHAEOGLOBUS FULGIDUS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 120141.16

構造登録者

Rodriguez, A.C.,Stock, D. (登録日: 2001-08-21, 公開日: 2002-02-11, 最終更新日: 2024-11-13)

主引用文献

Rodriguez, A.C.,Stock, D.
Crystal Structure of Reverse Gyrase: Insights Into the Positive Supercoiling of DNA.
Embo J., 21:418-, 2002

PubMed Abstract: Reverse gyrase is the only topoisomerase known to positively supercoil DNA. The protein appears to be unique to hyperthermophiles, where its activity is believed to protect the genome from denaturation. The 120 kDa enzyme is the only member of the type I topoisomerase family that requires ATP, which is bound and hydrolysed by a helicase-like domain. We have determined the crystal structure of reverse gyrase from Archaeoglobus fulgidus in the presence and absence of nucleotide cofactor. The structure provides the first view of an intact supercoiling enzyme, explains mechanistic differences from other type I topoisomerases and suggests a model for how the two domains of the protein cooperate to positively supercoil DNA. Coordinates have been deposited in the Protein Data Bank under accession codes 1GKU and 1GL9.

PubMed: 11823434
DOI: 10.1093/EMBOJ/21.3.418

実験手法

X-RAY DIFFRACTION (2.7 Å)