1GH6

RETINOBLASTOMA POCKET COMPLEXED WITH SV40 LARGE T ANTIGEN

Summary for 1GH6

• Entry DOI: 10.2210/pdb1gh6/pdb
• Descriptor: Large T antigen, Retinoblastoma-associated protein (2 entities in total)
• Functional Keywords: tumor suppressor, oncoprotein, antitumor protein
• Biological source: Simian virus 40 (SV40); More
• Cellular location: Host nucleus : P03070; Nucleus : P06400
• Total number of polymer chains: 2
• Total formula weight: 52463.49

Authors

Kim, H.Y.,Cho, Y. (deposition date: 2000-11-15, release date: 2001-11-15, Last modification date: 2023-08-09)

Primary citation

Kim, H.Y.,Ahn, B.Y.,Cho, Y.
Structural basis for the inactivation of retinoblastoma tumor suppressor by SV40 large T antigen.
EMBO J., 20:295-304, 2001

PubMed Abstract: Inactivation of the retinoblastoma (Rb) tumor suppressor by Simian virus 40 (SV40) large T antigen is one of the central features of tumorigenesis induced by SV40. Both the N-terminal J domain and the LxCxE motif of large T antigen are required for inactivation of Rb. The crystal structure of the N-terminal region (residues 7-117) of SV40 large T antigen bound to the pocket domain of Rb reveals that large T antigen contains a four-helix bundle, and residues from helices alpha2 and alpha4 and from a loop containing the LxCxE motif participate in the interactions with Rb. The two central helices and a connecting loop in large T antigen have structural similarities with the J domains of the molecular chaperones DnaJ and HDJ-1, suggesting that large T antigen may use a chaperone mechanism for its biological function. However, there are significant differences between large T antigen and the molecular chaperones in other regions and these differences are likely to provide the specificity needed for large T antigen to inactivate Rb.

PubMed: 11226179
DOI: 10.1093/emboj/20.1.295

Experimental method

X-RAY DIFFRACTION (3.2 Å)