1GDT

CRYSTAL STRUCTURE OF A SITE-SPECIFIC RECOMBINASE, GAMMA-DELTA RESOLVASE COMPLEXED WITH A 34 BP CLEAVAGE SITE

1GDT の概要

• エントリーDOI: 10.2210/pdb1gdt/pdb
• 分子名称: SITE I OF RES DNA, PROTEIN (GAMMA DELTA RESOLVASE), ... (5 entities in total)
• 機能のキーワード: protein-dna complex, double helix, overhanging base, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 61941.75

構造登録者

Yang, W.,Steitz, T.A. (登録日: 1995-04-11, 公開日: 1996-04-03, 最終更新日: 2024-02-07)

主引用文献

Yang, W.,Steitz, T.A.
Crystal structure of the site-specific recombinase gamma delta resolvase complexed with a 34 bp cleavage site.
Cell(Cambridge,Mass.), 82:193-207, 1995

PubMed Abstract: The structure of gamma delta resolvase complexed with a 34 bp substrate DNA has been determined at 3.0 A resolution. The DNA is sharply bent by 60 degrees toward the major groove and away from the resolvase catalytic domains at the recombination crossover point. The C-terminal one third of resolvase, which was disordered in the absence of DNA, forms an arm and a 3-helix DNA-binding domain on the opposite side of the DNA from the N-terminal domain. The arms wrap around the minor groove of the central 16 bp, and the DNA-binding domains interact with the major grooves near the outer boundaries of the binding site. The resolvase dimer is asymmetric, particularly in the arm region, implying a conformational adaptability that may be important for resolvase binding to different DNA sites in the synaptosome. It also raises the possibility of a sequential single-strand cleavage mechanism.

PubMed: 7628011
DOI: 10.1016/0092-8674(95)90307-0

実験手法

X-RAY DIFFRACTION (3 Å)