1GD6

STRUCTURE OF THE BOMBYX MORI LYSOZYME

1GD6 の概要

• エントリーDOI: 10.2210/pdb1gd6/pdb
• 分子名称: LYSOZYME (2 entities in total)
• 機能のキーワード: lysozyme, 1, 4-beta-n-acetylmuramidase, bmlz, hydrolase
• 由来する生物種: Bombyx mori (domestic silkworm)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13777.60

構造登録者

Matsuura, A.,Aizawa, T.,Yao, M.,Kawano, K.,Tanaka, I.,Nitta, K. (登録日: 2000-09-19, 公開日: 2001-03-21, 最終更新日: 2024-11-13)

主引用文献

Matsuura, A.,Yao, M.,Aizawa, T.,Koganesawa, N.,Masaki, K.,Miyazawa, M.,Demura, M.,Tanaka, I.,Kawano, K.,Nitta, K.
Structural analysis of an insect lysozyme exhibiting catalytic efficiency at low temperatures.
Biochemistry, 41:12086-12092, 2002

PubMed Abstract: Bombyx mori lysozyme (BmLZ), from the silkworm, is an insect lysozyme. BmLZ has considerable activity at low temperatures and low activation energies compared with those of hen egg white lysozyme (HEWLZ), according to measurements of the temperature dependencies of relative activity (lytic and glycol chitin) and the estimation of activation energies using the Arrhenius equation. Being so active at low temperatures and low activation energies is characteristic of psychrophilic (cold-adapted) enzymes. The three-dimensional structure of BmLZ has been determined by X-ray crystallography at 2.5 A resolution. The core structure of BmLZ is similar to that of c-type lysozymes. However, BmLZ shows some distinct differences in the two exposed loops and the C-terminal region. A detailed comparison of BmLZ and HEWLZ suggests structural rationalizations for the differences in the catalytic efficiency, stability, and mode of activity between these two lysozymes.

PubMed: 12356308
DOI: 10.1021/bi016099j

実験手法

X-RAY DIFFRACTION (2.5 Å)