1GCC

SOLUTION NMR STRUCTURE OF THE COMPLEX OF GCC-BOX BINDING DOMAIN OF ATERF1 AND GCC-BOX DNA, MINIMIZED AVERAGE STRUCTURE

Summary for 1GCC

• Entry DOI: 10.2210/pdb1gcc/pdb
• Descriptor: DNA (5'-D(*TP*AP*GP*CP*CP*GP*CP*CP*AP*GP*C)-3'), DNA (5'-D(*GP*CP*TP*GP*GP*CP*GP*GP*CP*TP*A)-3'), ETHYLENE RESPONSIVE ELEMENT BINDING FACTOR 1 (3 entities in total)
• Functional Keywords: transcription factor, protein-dna complex, ethylene inducible, complex (transcription factor-dna), transcription-dna complex, transcription/dna
• Biological source: Arabidopsis thaliana (thale cress)
• Cellular location: Nucleus (Probable): O80337
• Total number of polymer chains: 3
• Total formula weight: 13956.70

Authors

Yamasaki, K.,Allen, M.D.,Ohme-Takagi, M.,Tateno, M.,Suzuki, M. (deposition date: 1998-03-13, release date: 1999-03-23, Last modification date: 2024-10-23)

Primary citation

Allen, M.D.,Yamasaki, K.,Ohme-Takagi, M.,Tateno, M.,Suzuki, M.
A novel mode of DNA recognition by a beta-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA.
EMBO J., 17:5484-5496, 1998

PubMed Abstract: The 3D solution structure of the GCC-box binding domain of a protein from Arabidopsis thaliana in complex with its target DNA fragment has been determined by heteronuclear multidimensional NMR in combination with simulated annealing and restrained molecular dynamic calculation. The domain consists of a three-stranded anti-parallel beta-sheet and an alpha-helix packed approximately parallel to the beta-sheet. Arginine and tryptophan residues in the beta-sheet are identified to contact eight of the nine consecutive base pairs in the major groove, and at the same time bind to the sugar phosphate backbones. The target DNA bends slightly at the central CG step, thereby allowing the DNA to follow the curvature of the beta-sheet.

PubMed: 9736626
DOI: 10.1093/emboj/17.18.5484

Experimental method

SOLUTION NMR