1GA8

CRYSTAL STRUCTURE OF GALACOSYLTRANSFERASE LGTC IN COMPLEX WITH DONOR AND ACCEPTOR SUGAR ANALOGS.

1GA8 の概要

• エントリーDOI: 10.2210/pdb1ga8/pdb
• 関連するPDBエントリー: 1G9R
• 関連するBIRD辞書のPRD_ID: PRD_900074
• 分子名称: GALACTOSYL TRANSFERASE LGTC, 4-deoxy-beta-D-xylo-hexopyranose-(1-4)-beta-D-glucopyranose, MANGANESE (II) ION, ... (5 entities in total)
• 機能のキーワード: alpha-beta protein, transferase
• 由来する生物種: Neisseria meningitidis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37060.54

構造登録者

Persson, K.,Ly, H.D.,Diekelmann, M.,Wakarchuk, W.W.,Withers, S.G.,Strynadka, N.C.J. (登録日: 2000-11-29, 公開日: 2001-02-14, 最終更新日: 2024-10-16)

主引用文献

Persson, K.,Ly, H.D.,Dieckelmann, M.,Wakarchuk, W.W.,Withers, S.G.,Strynadka, N.C.
Crystal structure of the retaining galactosyltransferase LgtC from Neisseria meningitidis in complex with donor and acceptor sugar analogs.
Nat.Struct.Biol., 8:166-175, 2001

PubMed Abstract: Many bacterial pathogens express lipooligosaccharides that mimic human cell surface glycoconjugates, enabling them to attach to host receptors and to evade the immune response. In Neisseria meningitidis, the galactosyltransferase LgtC catalyzes a key step in the biosynthesis of lipooligosaccharide structure by transferring alpha-d-galactose from UDP-galactose to a terminal lactose. The product retains the configuration of the donor sugar glycosidic bond; LgtC is thus a retaining glycosyltranferase. We report the 2 A crystal structures of the complex of LgtC with manganese and UDP 2-deoxy-2-fluoro-galactose (a donor sugar analog) in the presence and absence of the acceptor sugar analog 4'-deoxylactose. The structures, together with results from site-directed mutagenesis and kinetic analysis, give valuable insights into the unique catalytic mechanism and, as the first structure of a glycosyltransferase in complex with both the donor and acceptor sugars, provide a starting point for inhibitor design.

PubMed: 11175908
DOI: 10.1038/84168

実験手法

X-RAY DIFFRACTION (2 Å)