1G85

CRYSTAL STRUCTURE OF BOVINE ODORANT BINDING PROTEIN COMPLEXED WITH IS NATURAL LIGAND

1G85 の概要

• エントリーDOI: 10.2210/pdb1g85/pdb
• 関連するPDBエントリー: 1OBP
• 分子名称: ODORANT-BINDING PROTEIN, (3R)-oct-1-en-3-ol (3 entities in total)
• 機能のキーワード: lipocalin, swapping domain, homodimer, signaling protein
• 由来する生物種: Bos taurus (cattle)
• 細胞内の位置: Secreted: P07435
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 37425.48

構造登録者

Vincent, F.,Spinelli, S.,Cambillau, C.,Tegoni, M. (登録日: 2000-11-16, 公開日: 2002-06-26, 最終更新日: 2023-08-09)

主引用文献

Ramoni, R.,Vincent, F.,Grolli, S.,Conti, V.,Malosse, C.,Boyer, F.D.,Nagnan-Le Meillour, P.,Spinelli, S.,Cambillau, C.,Tegoni, M.
The insect attractant 1-octen-3-ol is the natural ligand of bovine odorant-binding protein.
J.Biol.Chem., 276:7150-7155, 2001

PubMed Abstract: Bovine odorant-binding protein (bOBP) is a dimeric lipocalin present in large amounts in the respiratory and olfactory nasal mucosa. The structure of bOBP refined at 2.0-A resolution revealed an elongated volume of electron density inside each buried cavity, indicating the presence of one (or several) naturally occurring copurified ligand(s) (Tegoni et al. (1996) Nat. Struct. Biol. 3, 863-867; Bianchet et al. (1996) Nat. Struct. Biol. 3, 934-939). In the present work, by combining mass spectrometry, x-ray crystallography (1.8-A resolution), and fluorescence, it has been unambiguously established that natural bOBP contains the racemic form of 1-octen-3-ol. This volatile substance is a typical component of bovine breath and in general of odorous body emanations of humans and animals. The compound 1-octen-3-ol is also an extremely potent olfactory attractant for many insect species, including some parasite vectors like Anopheles (Plasmodium) or Glossina (Trypanosoma). For the first time, a function can be assigned to an OBP, with a possible role of bOBP in the ecological relationships between bovine and insect species.

PubMed: 11114310
DOI: 10.1074/jbc.M010368200

実験手法

X-RAY DIFFRACTION (1.8 Å)